Bacterial diversity in human subgingival plaque. J Bacteriol 2001;183:3770-83. 32. Socransky SS, Haffajee AD. Dental biofilms: difficult therapeutic targets.

2017-06-02 · The subgingival plaque was collected by inserting a sterile dental curette or sterile paper points into the bottom of the gingival crevice . The primary outcome measure was the frequency of periodontopathogen in the subgingival plaques, which referred to the percentage of the patients or teeth positive for periodontopathogens.

Subgingival plaque was frequently characterized by a zone of gram negative and/or motile species located adjacent to the epithelial lining of the pocket while gram positive 2016-01-01 Background: The collection of subgingival plaque samples with paper points is time‐consuming and accident‐sensitive. However, the collection of saliva is simple and contains pathogens of all intraoral surfaces. The aim of this study is to investigate whether a sampling strategy with mouthrinse (mouthrinse sample [MSP]; test) leads to results 2015-11-27 2010-07-01 2017-04-03 Subgingival plaque sample collection. For the diseased samples, the deepestthreepocketswereselectedandpooled.Supragingivalplaquewas ﬁrst removed from the sample teeth with sterilized Gracey curettes and gauze.

Diagrammatic model of supragingival plaque collected after 0, 2, 4 and 6 hours of plaque formation. 17 May 2018 Collection of subgingival biofilms and saliva. Subgingival plaque samples were collected as described in [35]. In brief, four subgingival biofilm dental plaque biofilm; periodontitis; host defense mechanisms; resistance microbiota, accompanied by a diverse collection of archaea, fungi, protozoa, and viruses. The specific composition of subgingival bacterial plaque in gingiv 10 Jun 2015 subgingival plaques of Algerian healthy adults. Supragingival and subgingival plaque samples were collected from 65 Algerian caries-free, for diagnostic quantitative anaerobic culture of subgingival plaque samples.

Technically, local environmental changes related to orthodontic band and brackets may influence the bacterial species in periodontal plaque. However, it seems necessary to assess variations in subgingival plaque caused by orthodontic appliances. The aim of this study was to investigate changes in 2021-01-15 Pooled subgingival plaque samples were collected and analyzed for detection of bacteria and viruses using loop‐mediated isothermal amplification.

Prevalence of Candida species in subgingival plaque samples was quantified as 73.3% in Group 1, 66.6% in Group 2 and 60% in Group 3 and no statistically significant differences were observed between groups. Candida albicans was the most frequently isolated species followed by Candida krusei and Candida tropicalis.

1 May 2018 Twenty-one subgingival plaque samples were collected from three patients with chronic periodontitis (ChP), three patients with aggressive Detecting the periodontal pathogens at the subgingival plaque requires skilled professionals to collect samples. Periodontal pathogens are also detected on 3 Apr 2017 Four samples (subgingival and supragingival plaques, saliva, and tongue coating) per each subject were collected from 14 patients with a results dependent on the subgingival biofilm collection method. Subgingival biofilm plaque. Oral Microbiol Immunol 2008 23: 196-205.

Collection of subgingival plaques Subgingival plaquesampleswerecollectedby asterile hand scalerandtransferred ineither one ml of sterile reduced transfer ﬂuid (RTF) consisting of 0.01% dithiothreitol in PBS, pH 7.4 or one ml of distilled water. The samples were immediately placed at 4 C and analyzed within 4 h.

However, the collection of saliva is simple and contains pathogens of all intraoral surfaces. The aim of this study is to investigate whether a sampling strategy with mouthrinse (mouthrinse sample [MSP]; test) leads to results A total of 1,170 samples of supra and subgingival plaque were collected from the mesial aspect of every tooth (up to 28 supra and 28 subgingival samples) from each subject and evaluated for the plastic tubes during the final 5 min. Supragingival plaque samples were collected and pooled by sterile curettes from all teeth surfaces (6.5 ± 0.9 teeth), on the side of the upper half-jaw that contained the most teeth.

The site was then cleaned and isolated using cotton roles and air dried gently. The collection of subgingival plaque samples is the common way for the determination of periodontopathic bacteria. However, recently, salivary analysis has been discussed as an advantageous future diagnostic method for periodontitis because it offers simple quantitative sampling and the possibility to assess various bacteria.
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Define subgingival plaque. subgingival plaque synonyms, subgingival plaque pronunciation, subgingival plaque translation, English dictionary definition of subgingival plaque. n. 1.

Supragingival plaque was first removed from the sample teeth with sterilized Gracey curettes and gauze.
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Subgingival plaque was analyzed from healthy subjects and subjects with refractory periodontitis, adult periodontitis, human immunodeficiency virus periodontitis, and acute necrotizing ulcerative gingivitis. 16S ribosomal DNA (rDNA) bacterial genes from DNA isolated from subgingival plaque samples were PCR amplified with all-bacterial or selective primers and cloned into Escherichia coli .

Oshrain HI, Salind A, Mandel ID. PMID: 5247121 [Indexed for MEDLINE] MeSH terms.